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Mouse IgG (H&L) Antibody Texas Red Conjugated

Catalog Number: orb347635

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb347635
CategoryAntibodies
DescriptionMouse IgG (H&L) antibody (Texas Red)
Species/HostDonkey
ClonalityPolyclonal
Tested applicationsDOT, FC, FLISA, IF, WB
ReactivityMouse
IsotypeIgG
ImmunogenMouse IgG whole molecule
Concentration2.0 mg/mL
Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Form/AppearanceLyophilized
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.
ConjugationTexas Red
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesDonkey anti-Mouse IgG Texas Red Conjugated Antibod
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NoteFor research use only
Application notesAnti-Mouse IgG Texas Red Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Expiration Date12 months from date of receipt.
Mouse IgG (H&L) Antibody Texas Red Conjugated

Western Blot of Texas Red™ Donkey Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red™ donkey secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.

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