Mouse IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

• Catalog Number: orb347504
• Category: Antibodies
• Description: Mouse IgG (H&L) antibody (TRITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
• Conjugation: TRITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit Anti-Mouse IgG Antibody rhodamine Conjugati
• Note: For research use only
• Application notes: Anti-Mouse IgG Rhodamine Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Anti Mouse IgG (Rabbit) mx Hu-Rhodamine conjugated. Lane 1: 100 ng of Ms IgG. Lane 2-5: serial dilution 3 fold starting at 100 ng. Primary Antibody: none. Secondary Antibody: Rabbit Anti-Mouse IgG mx Hu-Rhodamine Conj'd at 1 mg/ml at RT for 30 minutes. Block: orb348637 at RT for 30 minutes.