Mouse IgG (H&L) Antibody Rhodamine Conjugated

• Catalog Number: orb347494
• Category: Antibodies
• Description: Mouse IgG (H&L) antibody (TRITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG, and Mouse Serum.
• Conjugation: TRITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit Anti-Mouse IgG Antibody rhodamine Conjugati
• Note: For research use only
• Application notes: Anti-Mouse IgG Rhodamine Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Fluorescence-activated cell sorting analysis after co-icubation of HCT116 cells with FITC-labeled CaIX-P1 and rhodamine-labeled anti-human CAIX mAb. Low left box: unlabeled cells (autofluorescence). Low right box: cells labeled only with FITC-CaIX-P1. Upper left box: cells labeled only with rhodamine-anti-CAIX-mAb. Upper right box: Cells labeled with both FITC-CaIX-P1 and rhodamine-anti-CAIX-mAb.

Western blot of Rhodamine conjugated Rabbit Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.