Mouse IgG (H&L) antibody (Peroxidase)

• Catalog Number: orb347619
• Category: Antibodies
• Description: Mouse IgG (H&L) antibody (Peroxidase)
• Species/Host: Sheep
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Anti-Mouse IgG [H&L] was produced by repeated immunization with Mouse IgG whole molecule in sheep.
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:100,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Sheep Anti-Mouse IgG[H&L] Peroxidase Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Mouse IgG and Mouse Serum.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: sheep anti-Mouse IgG heavy and light chain peroxid
• Note: For research use only
• Application notes: Anti-Mouse IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Western Blot of Peroxidase conjugated Sheep anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase sheep secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Other band(s): none.