Mouse IgG (H&L) antibody

• Catalog Number: orb347415
• Category: Antibodies
• Description: Mouse IgG (H&L) antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, FLISA, IF, IHC, IP, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
• Concentration: 2.1 mg/ml
• Dilution range: ELISA: 1:25,000, FLISA: User Optimized, FC: User Optimized, IHC: 1:1,000 - 1:5,000, IF: User Optimized, IP: User Optimized, WB: 1:3,000 - 1:15,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
• Conjugation: Unconjugated
• Storage: Store Anti-Mouse Secondary Antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat anti-Mouse IgG Secondary Antibody, Mouse Seco
• Note: For research use only
• Application notes: Anti-Mouse IgG affinity purified antibody is generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse IgG secondary antibody has been tested by ELISA and western blot and is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Goat Anti-Mouse IgG Antibody tested against purified Mouse IgG protein. Each well was coated in duplicate with 1.0 µg of Mouse IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:35800. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti-Goat IgG Peroxidase Conjugate at 1:40000 and TMB substrate p/n orb348651.

Western Blot of Goat anti-Mouse IgG (H&L) Antibody. Lane 1: Mouse IgG. Load: 100 ng per lane. Primary Antibody: Mouse IgG (H&L) Antibody 1:1000 overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed Size: 28 and 55 kDa/28 and 55 kDa for Mouse IgG. Other band(s): none.

Western Blot of Goat Anti-Mouse IgG (H&L) Antibody. Lane M: Molecular Ladder. Lane 1: Mouse IgG whole molecule. Lane 2: Mouse IgG F(c) Fragment (p/n orb346280). Lane 3: Mouse IgG F(ab) Fragment (orb346282). Lane 4: Mouse IgM Kappa (p/n orb343770). Lane 5: Mouse Serum. Load: 50 ng per lane. Block: (p/n orb348637) for 30 min at RT. Primary Antibody: Anti-Mouse IgG (H&L) Antibody 1:1000 for 60 min at RT. Secondary antibody: HRP Donkey Anti-Goat IgG (p/n orb347032) at 1:40000 for 30 min at RT. Predicted/Observed Size: 28 and 55 kDa for Mouse IgG, F(c), F(ab), IgM Kappa, and Serum. Mouse F(c) migrates slightly higher.