Mouse IgG F(c) Biotin Antibody

• Catalog Number: orb346295
• Category: Proteins
• Description: Mouse IgG F(c) Biotin Antibody
• Tested applications: DOT
• Isotype: IgG F(c)
• Concentration: 1.0 mg/mL
• Form/Appearance: Lyophilized
• Purity: This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain.
• Conjugation: Biotin
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Mouse IgG F(c) Fragment Biotin Conjugation, Mouse
• Note: For research use only
• Application notes: Mouse IgG F(c) fragment has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Mouse IgG F(c) Fragment Biotin Conjugated. Row: Mouse IgG F(c) Biotin Conjugate [1.5 mg/mL]. Column Dilutions: 100 ng, 33.33 ng, 11.11 ng, 3.70 ng, 1.23 ng. Primary Antibody: None. Secondary Antibody: Streptavidin Peroxidase (p/n orb348767) at 1:40000 for 30 mins at RT. Block: BlockOut buffer (p/n orb348644). Exposure: 2 sec.

SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 1: 5 µL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 3: Reduced Mouse F(c) Fragment (p/n orb346280). Lane 4: Reduced Mouse F(ab) Fragment (p/n orb346282). Lane 5: Mouse IgM Kappa Myeloma Protein (p/n orb346283). Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.