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Catalog Number | orb346280 |
---|---|
Category | Proteins |
Description | Mouse IgG F(c) Antibody |
Tested applications | ELISA, IHC, SDS-PAGE, WB |
Isotype | IgG F(c) |
Concentration | 1.1 mg/mL |
Dilution range | ELISA: User Optimized, IHC: User Optimized, WB: User Optimized |
Form/Appearance | Liquid (sterile filtered) |
Purity | MOUSE IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F(c). No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain. |
Conjugation | Unconjugated |
Source | Mouse |
Biological Origin | Mouse |
Storage | Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Mouse IgG Fc fragment is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Alternative names | MOUSE IgG F(c) fragment, Mouse Fc, Immunoglobulin Read more... |
Note | For research use only |
Application notes | Mouse IgG F(c) Fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 1: 5 µL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 3: Reduced Mouse F(c) Fragment (p/n orb346280). Lane 4: Reduced Mouse F(ab) Fragment (p/n orb346282). Lane 5: Mouse IgM Kappa Myeloma Protein (p/n orb346283). Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
DOT, ELISA, IHC, WB | |
Mouse | |
Rabbit | |
Polyclonal | |
AP |
DOT | |
Biotin | |
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain. | |
Mouse |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain. | |
Mouse |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain. | |
Mouse |
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