You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb346282 |
---|---|
Category | Proteins |
Description | Mouse IgG Fab fragment Antibody |
Tested applications | ELISA, IHC, SDS-PAGE, WB |
Isotype | IgG Fab |
Concentration | 2.0 mg/mL |
Dilution range | ELISA: User Optimized, IHC: User Optimized, WB: User Optimized |
Form/Appearance | Liquid (sterile filtered) |
Purity | Mouse IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Mouse IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG, anti-Mouse IgG F(ab’)2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti- Papain. |
Conjugation | Unconjugated |
Source | Mouse |
Biological Origin | Mouse |
Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Mouse Immunoglobulin Fab, F(ab), Fragment antigen- Read more... |
Note | For research use only |
Application notes | Mouse IgG Fab Fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 1: 5 µL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 3: Reduced Mouse F(c) Fragment (p/n orb346280). Lane 4: Reduced Mouse F(ab) Fragment (p/n orb346282). Lane 5: Mouse IgM Kappa Myeloma Protein (p/n orb346283). Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
Filter by Rating