Mouse IgG Fab Biotin Antibody

• Catalog Number: orb346297
• Category: Proteins
• Description: Mouse IgG Fab Biotin Antibody
• Isotype: IgG Fab
• Concentration: 1.0 mg/mL
• Form/Appearance: Lyophilized
• Purity: This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(ab’)2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti-Papain.
• Conjugation: Biotin
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Mouse IgG Fab Fragment Biotin Conjugation
• Note: For research use only
• Application notes: Mouse IgG Fab Fragment Biotin can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). MW: 5 µl Opal Prestained Marker. Lane 1: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 2: Reduced Mouse F(c) Fragment (p/n orb346280). Lane 3: Reduced Mouse F(ab) Fragment (p/n orb346282). Lane 4: Mouse IgM Kappa Myeloma Protein. Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.