Mouse IgA Kappa isotype Control Antibody

• Catalog Number: orb343771
• Category: Proteins
• Description: Mouse IgA Kappa isotype Control Antibody
• Tested applications: ELISA, FC, FLISA, IF, SDS-PAGE, WB
• Isotype: IgA
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: User Optimized, FLISA: User Optimized, FC: 1:1000-1:5000, IF: User Optimized, WB: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: Mouse IgA Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgA alpha and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3, IgM. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.
• Conjugation: Unconjugated
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: Mouse IgA istoype control, Mouse IgA subclass isto
• Note: For research use only
• Application notes: Mouse IgA kappa isotype control has been tested in ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
• Expiration Date: 12 months from date of receipt.

SDS PAGE Results of Mouse IgA kappa Isotype Control. Lane 1: Mouse IgA kappa Isotype Control Reduced [1 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Mouse IgA kappa Isotype Control Non-Reduced [1 µg]. 4-20% Gel, Coomassie Stained.