Mouse HbA1c ELISA Kit

• Catalog Number: orb778970
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glycated Hemoglobin A1c(HbA1c) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycated Hemoglobin A1c(HbA1c).
• Tested applications: ELISA
• Reactivity: Mouse
• Range: 15.63-1000 μg/mL
• Sensitivity: 4.55 μg/mL
• Protein Length: 2h
• Alternative names: Glycosylated Hemoglobin ELISA Kit, Hemoglobin A1c
• Note: For research use only
• Application notes: Standard: 1000 μg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse HbA1c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: plasma and erythrocyte lysates
• Expiration Date: Please enquire.