Mouse FGF basic protein

• Catalog Number: orb80024
• Category: Proteins
• Description: Mouse FGF basic protein
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: Mouse FGF basic
• Solubility (25°C): It is recommended to reconstitute the lyophilized Fibroblast Growth Factor in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be Met-Pro-Ala-Leu-Pro-Glu.
• Biological Origin: E.coli
• Biological Activity: The ED50 range= 0.1ng/ml, determined by the dose-dependant proliferation of BALB/3T3 cells.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: FGF-b was lyophilized from 5mM NaP, pH7.5 and 50mM NaCl.
• Alternative names: HBGH-2 protein, HBGF-2 protein, Prostatropin prote
• Note: For research use only
• Application notes: Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.885 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of FGF2 as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Mouse FGF basic protein