(Mouse) Epcam Antibody (C-term)

• Catalog Number: orb1788010
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 302-335 aa
• Dilution range: WB: 1:1000, FC: 1:25
• Conjugation: Unconjugated
• MW: 35019 Da
• Target: This Mouse Epcam antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 302-335 amino acids from the C-terminal region of mouse Epcam.
• UniProt ID: Q99JW5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Epithelial cell adhesion molecule, Ep-CAM, Epithel
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of HepG2 cells using (Mouse) Epcam Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

All lanes: Anti-Epcam Antibody (C-term) at 1:1000 dilution. Lane 1: mouse kidney lysates. Lane 2: mouse lung lysates. Lane 3: mouse testis lysates. Lane 4: MCF-7 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.