Mouse Csf1r Antibody (C-term)

• Catalog Number: orb1936300
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB34462
• Tested applications: IF, IHC-P, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:25, WB: 1:1000-2000, WB: 1:1000, WB: 1:1000, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 109179 Da
• Target: This Mouse Csf1r antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 895-923 amino acids from the C-terminal region of mouse Csf1r.
• UniProt ID: P09581
• NCBI: NP_001032948.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Macrophage colony-stimulating factor 1 receptor, C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Mouse Csf1r Antibody (C-term) western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the Csf1r antibody detected the Csf1r protein (arrow).

All lanes: Anti-Mouse Csf1r Antibody (C-term) at 1:1000 dilution. Lane 1: Mouse heart lysate. Lane 2: Mouse skeletal muscle lysate. Lane 3: Rat skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 109 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Mouse Csf1r Antibody (C-term) at 1:1000-2000 dilution. Lane 1: Mouse heart tissue lysate. Lane 2: Mouse skeletal muscle tissue lysate. Lane 3: Mouse spleen tissue lysate. Lane 4: Rat brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 109 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labeling Csf1r at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm staining on NIH/3T3 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (red). The nuclear counter stain is DAPI (blue).

Staining Mouse Csf1r in mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.