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Catalog Number | orb359104 |
---|---|
Category | Proteins |
Description | Recombinant mouse CCL3 active protein |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | > 98% as determined by SDS-PAGE and HPLC. |
MW | 7.9 kDa |
UniProt ID | P10855 |
Protein Sequence | APYGADTPTACCFSYSRKIPRQFIVDYFETSSLCSQPGVIFLTKRNRQICADSKETWVQEYITDLELNA |
Protein Length | Full Length of Mature Protein |
Source | E.Coli |
Biological Origin | Mus musculus (Mouse) |
Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using murine splenocytes is in a concentration range of 10-100 ng/ml. |
Expression Region | 24-92aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA |
Alternative names | Heparin-binding chemotaxis protein, Macrophage inf Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
7.9 kDa (Predicted) |
> 95% by SDS-PAGE and HPLC analyses. | |
Approximately 11.5 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids. | |
Escherichia coli. |
> 95% as analyzed by SDS-PAGE and HPLC. | |
7.8 kDa, observed by reducing SDS-PAGE. | |
HEK 293 |