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Catalog Number | orb1926941 |
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Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB43270 |
Tested applications | WB |
Reactivity | Mouse |
Isotype | Rabbit IgG |
Dilution range | WB: 1:1000, WB: 1:2000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 74000 Da |
Target | This MOUSE Brd7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 387-418 amino acids from the Central region of human MOUSE Brd7. |
UniProt ID | O88665 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Bromodomain-containing protein 7, 75 kDa bromodoma Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysate from mouse lung tissue lysate, using MOUSE Brd7 Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35 ug.
All lanes: Anti-Brd7 Antibody (Center) at 1:2000 dilution. Lane 1: mouse heart lysate. Lane 2: mouse kidney lysate. Lane 3: mouse lung lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 74 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.