Mouse APP ELISA Kit

• Catalog Number: orb778136
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amyloid Precursor Protein(APP).
• Tested applications: ELISA
• Reactivity: Mouse
• Range: 0.32-20 ng/mL
• Concentration: 20 ng/mL
• UniProt ID: P12023
• Sensitivity: 0.117 ng/mL
• Protein Length: 3.5h
• Alternative names: AAA ELISA Kit, ABeta ELISA Kit, ABPP ELISA Kit, AD
• Note: For research use only
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse APP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APP in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Expiration Date: Please enquire.