Mouse anti Human HLA Class I Heavy Chain (Restricted expression)

• Catalog Number: orb137660
• Category: Antibodies
• Description: HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HC10
• Tested applications: ELISA, FC, IA, ICC, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG2a
• Form/Appearance: Each vial contains 100 µl 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Conjugation: Unconjugated
• Source: HC10 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Each vial contains 100 µl 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunostaining of human tonsil tissue in paraffin section with HC10 (dilution 1:20.000).