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Catalog Number | orb198166 |
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Category | Antibodies |
Description | Mouse polyclonal antibody to CD4, CD27 (FITC, PE) |
Species/Host | Mouse |
Tested applications | FACS |
Reactivity | Human |
Isotype | IgG1 (F)/IgG1 (PE) |
Immunogen | CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes. |
Concentration | Titered for flow cytometry |
Form/Appearance | Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein |
Conjugation | FITC/RPE |
Hazard Information | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. |
UniProt ID | P26842, P01730 |
Storage | Product should be stored at 4-8°C. DO NOT FREEZE |
Buffer/Preservatives | Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein |
Alternative names | CD4 FITC - CD27 PE Read more... |
Note | For research use only |
Application notes | PBMC: Add10 µl of MAB/106^ PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
Expiration Date | 12 months from date of receipt. |