Mouse anti Human CD4 FITC - CD8 PE

• Catalog Number: orb198131
• Category: Antibodies
• Description: Mouse polyclonal antibody to CD4, CD8 (FITC, PE)
• Species/Host: Mouse
• Tested applications: FACS
• Reactivity: Human
• Isotype: IgG1 (F)/IgG1(PE)
• Immunogen: CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
• Concentration: Titered for flow cytometry
• Form/Appearance: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Conjugation: FITC/RPE
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• UniProt ID: P01730, P01732
• Storage: Product should be stored at 4-8°C. DO NOT FREEZE
• Buffer/Preservatives: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Alternative names: CD4 FITC - CD8 PE
• Note: For research use only
• Application notes: PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Peripheral Blood Lymphocytes using CD4, CD8 antibody (FITC, PE)