Mouse Anti-Bovine IgG (L chain) antibody

• Catalog Number: orb44871
• Category: Antibodies
• Description: Mouse Monoclonal to Bovine IgG (L chain).
• Clonality: Monoclonal
• Clone Number: IVA285-1
• Tested applications: FC, IHC-Fr, WB
• Reactivity: Bovine, Sheep
• Isotype: Mouse IgG1
• Immunogen: Fraction of Bovine immunoglobulin.
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 0,5-1 μg/ml, overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Ig Light Chains
• RRID: AB_10990179
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-IgG light chain antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 0,5-1 μg/ml, overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Expiration Date: 12 months from date of receipt.

Western blotting detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and Bovine Serum (right lane) by antibody IVA285-1. This antibody is a suitable tool for in-house quality controls of Fetal Calf Serum (Hybridoma Cell Culture grade).

Separation of bovine Ig Light Chains positive leukocytes (red-filled) from Ig Light Chains negative leukocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine Ig Light Chains (IVA285-1) purified antibody (concentration in sample 3 µg/ml, GAM APC).

Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine Ig Light Chains (IVA285-1) purified antibody (concentration in sample 3 µg/ml, GAM APC).