MORC3 antibody

• Catalog Number: orb750553
• Category: Antibodies
• Description: MORC3 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse
• Isotype: Antiserum
• Immunogen: Morc3 whole rabbit serum was prepared by repeated immunizations with a human Morc3 recombinant protein.
• Concentration: 70 mg/mL
• Dilution range: ELISA: 1:20,000-1:50,000, WB: 1:1000-1:5000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Morc3 antibody was prepared from monospecific antiserum by delipidation and defibrination. The antibody is specific for human Morc3 in expressed cell lysates. Cross reactivity is seen in mouse Morc3. Cross reactivity to other Morc proteins has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q14149
• NCBI: NP_056173.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Morc3 Antibody, MORC family CW-type zi
• Note: For research use only
• Application notes: Anti-Morc3 Antibody is tested for use in Western Blot and suitable for ChIP and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 107.1 kDa in size corresponding Morc3 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Morc3 antibody. Lane 1: Human embryonic stem cell. Lane 2: Human embryonic stem cell. Lane 3: C-Flag Mouse embryonic stem cell. Lane 4: C-Flag Mouse embryonic stem cell doxycycline induced. Load: 35 µg per lane. Primary antibody: hMorc3 antibody at 1:1000-1:5000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 107 kDa/ ~170 kDa. Other band(s): sumoylated Morc run higher.