MORC3 antibody

• Catalog Number: orb750552
• Category: Antibodies
• Description: MORC3 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse
• Isotype: Antiserum
• Immunogen: Morc3 whole rabbit serum was prepared by repeated immunizations with a human Morc3 recombinant protein.
• Concentration: 70 mg/mL
• Dilution range: ELISA: 1:20,000-1:50,000, WB: 1:1000-1:5000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Morc3 antibody was prepared from monospecific antiserum by delipidation and defibrination. The antibody is specific for human Morc3 in expressed cell lysates. Cross reactivity is seen in mouse Morc3. Cross reactivity to other Morc proteins has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q14149
• NCBI: NP_056173.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Morc3 Antibody, MORC family CW-type zi
• Note: For research use only
• Application notes: Anti-Morc3 Antibody is tested for use in Western Blot and suitable for ChIP and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 107.1 kDa in size corresponding Morc3 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Morc3 antibody. Lane 1: Human embryonic stem cell. Lane 2: Human embryonic stem cell. Lane 3: C-Flag Mouse embryonic stem cell. Lane 4: C-Flag Mouse embryonic stem cell doxycycline induced. Load: 35 µg per lane. Primary antibody: hMorc3 antibody at 1:1000-1:5000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 107 kDa/ ~170 kDa. Other band(s): sumoylated Morc run higher.