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Catalog Number | orb137140 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to MonoMethyl-Histone H3-K27 |
Clonality | Polyclonal |
Tested applications | ChIP, DOT, ICC, IF, IHC, WB |
Reactivity | All, Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic monomethylated peptide around K27 of human histone H3(NP_003520.1). |
Concentration | 1 mg/ml |
Dilution range | WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200, IP: 1:50-200, ChIP: 1:20-100, ChIP |
Conjugation | Unconjugated |
MW | 17KDa |
UniProt ID | P68431 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
Alternative names | anti HIST1H3J antibody, anti H3/j antibody, anti H Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human kidney cancer tissue using Histone Histone H3K27me1 antibody (primary antibody dilution at 1:200)
Immunofluorescense analysis of 293T cell using Histone Histone H3K27me1 antibody
Dot-blot analysis of all sorts of methylation peptides using H3K27me1 antibody
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli using Histone Histone H3K27me1 antibody
Immunohistochemical staining of paraffin embedded rat brain tissue using Histone Histone H3K27me1 antibody (primary antibody dilution at 1:200)
IHC-P image of human thyroid cancer tissue using Histone Histone H3K27me1 antibody (primary antibody dilution at 1:200)
IHC-P staining of rat lung tissue using Histone Histone H3K27me1 antibody (primary antibody dilution at 1:200)
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