Monoamine Oxidase B Polyclonal Antibody

• Catalog Number: orb1421185
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Monoamine Oxidase B.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat
• Conjugation: Unconjugated
• UniProt ID: P27338
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Amine oxidase [flavin-containing] B, 1.4.3.4, Mono
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Raji Cell (Human) Lysate at 40 ug. Primary: Anti-Monoamine Oxidase B at 1/300 dilution. Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution. Predicted band size: 59 kD. Observed band size: 59 kD.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Monoamine Oxidase B) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Monoamine Oxidase B) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.