Monkey IgM (mu chain) antibody (Alkaline Phosphatase)

• Catalog Number: orb348022
• Category: Antibodies
• Description: Monkey IgM (mu chain) antibody (Alkaline Phosphatase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Monkey
• Isotype: IgG
• Immunogen: Monkey IgM whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Monkey IgM (mu chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Monkey IgM and Monkey Serum. No reaction was observed against other Monkey heavy or light chain proteins.
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat Anti-Monkey IgM Antibody alkaline phosphatase
• Note: For research use only
• Application notes: Anti-Monkey IgM (mu chain) Alkaline Phosphatase Conjugate Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

This table displays additional reactivity among various species of serum and immunoglobulin. A (+) indicates antibody reactivity to the corresponding target.

Western Blot of Alkaline Phosphatase Conjugated Goat Anti-Monkey IgM (mu chain) Secondary Antibody. Lane 1: Monkey IgM. Lane 2: None. Load: 100 ng per lane. Primary antibody: None. Secondary antibody: Alkaline Phosphatase goat secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 72 kDa, 72 kDa for Monkey IgM. Other band(s): Monkey IgM splice variants and isoforms.