Monkey IgM (mu chain) antibody

• Catalog Number: orb348010
• Category: Antibodies
• Description: Monkey IgM (mu chain) antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Monkey
• Isotype: IgG
• Immunogen: Monkey IgM whole molecule
• Concentration: 1.05 mg/mL
• Dilution range: ELISA: 1:16,700 - 1:17,700, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgM and Monkey Serum. No reaction was observed against other monkey heavy or light chain proteins.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: Goat Anti-Monkey IgM mu chain Antibody, Goat Anti-
• Note: For research use only
• Application notes: Anti-Monkey IgM (mu chain) has been tested by ELISA, SDS-PAGE, dot blot, and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

This table displays additional reactivity among various species of serum and immunoglobulin. A (+) indicates antibody reactivity to the corresponding target.

Western Blot of Goat anti-Monkey IgM (mu chain) antibody. Lane 1: Monkey IgM (p/n orb346440). Lane 2: Monkey IgG. Lane 3: Monkey IgG (A and M Free). Lane 4: Monkey IgG F(c). Load: 50 ng per lane. Primary antibody: Goat anti-Monkey IgM (mu chain) antibody at 1:1000 for 1 hour at RT. Secondary antibody: Peroxidase conjugated Dk-a-Gt IgG at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 70 kDa, 70 kDa for Monkey IgM (mu chain).