Monkey IgG gamma antibody

• Catalog Number: orb348011
• Category: Antibodies
• Description: Monkey IgG gamma antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Monkey
• Isotype: IgG
• Immunogen: Anti-Monkey IgG (gamma chain) was produced by repeated immunization with monkey IgG gamma heavy chain in goat.
• Concentration: 1.26 mg/mL
• Dilution range: ELISA: 1:75000 - 1:175,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgG and Monkey Serum.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: goat anti-Monkey IgG (gamma chain) antibody
• Note: For research use only
• Application notes: Anti-Monkey IgG (gamma chain) has been tested by ELISA, dot blot, and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

This table displays additional reactivity among various species of serum and immunoglobulin. A (+) indicates antibody reactivity to the corresponding target.

Western Blot of Goat anti-Monkey IgG gamma Antibody. Lane 1: Monkey IgG. Lane 2: None. Load: 100 ng per lane. Primary antibody: Monkey IgG gamma Antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Monkey IgG Gamma. Other band(s): None.