Moesin Antibody

• Catalog Number: orb639524
• Category: Antibodies
• Description: Moesin (for membrane-organizing extension spike protein) is a member of the ERM protein family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [Wiki]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EDLP70
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Moesin antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Moesin antibody is available for research use only.
• UniProt ID: P26038
• Storage: Store the Moesin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Moesin antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human Jurkat cell lysate with Moesin antibody (clone EDLP70). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.

IHC testing of FFPE human melanoma with Moesin antibody (clone EDLP70). HIER: steam section in pH6 citrate buffer for 20 min.

IHC testing of FFPE human placenta with Moesin antibody (clone EDLP70). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry testing of human K562 cells with Moesin antibody (clone EDLP70); Red=isotype control, Blue= Moesin antibody.