MMP3 Antibody / Matrix Metalloproteinase 3

• Catalog Number: orb639812
• Category: Antibodies
• Description: The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also designated stromelysin-1, 2 and 3, respectively) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MMP3/2806
• Tested applications: ELISA, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A recombinant full length human protein was used as the immunogen for this MMP3 antibody.
• Dilution range: ELISA (order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This MMP3 antibody is available for research use only.
• UniProt ID: P08254
• Storage: Store the MMP3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human placenta with MMP3 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing. IHC staining of FFPE human placenta with MMP3 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using MMP3 antibody (clone MMP3/2806). These results demonstrate the foremost specificity of the MMP3/2806 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free MMP3 antibody (clone MMP3/2806) as confirmation of integrity and purity.