MMP2 Antibody

• Catalog Number: orb1825290
• Category: Antibodies
• Description: It recognizes a protein of 72kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MMP2/4586
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the Matrix metalloproteinase 2 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Matrix metalloproteinase 2 antibody is available for research use only.
• UniProt ID: P08253
• Storage: Aliquot the Matrix metalloproteinase 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human placental tissue with Matrix metalloproteinase 2 antibody (clone MMP2/4586). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Matrix metalloproteinase 2 antibody (clone MMP2/4586) as confirmation of integrity and purity.