MMP14 Antibody

• Catalog Number: orb1263948
• Category: Antibodies
• Description: MMP14 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This MMP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the N-terminal region of human MMP14.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 66 kDa
• Target: MMP14
• UniProt ID: P50281
• NCBI: P50281
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Matrix metalloproteinase-14, MMP-14, MMP-X1, Membr
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Antibody staining MMP14 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: mouse lung lysate Lane 4: MCF-7 whole cell lysate Lane 5: NCI-H292 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in NCI-H292 cell line lysates (35 ug/lane).This demonstrates the MMP14 antibody detected the MMP14 protein (arrow).

Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.