MMP14 Antibody

• Catalog Number: orb1258119
• Category: Antibodies
• Description: MMP14 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human MT1-MMP
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 46-70kDa
• Target: MMP14
• UniProt ID: P50281
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: MMP14, matrix metallopeptidase 14 (membrane-insert
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using MT1-MMP antibody (orb1258119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using MT1-MMP antibody (orb1258119) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney using MT1-MMP antibody (orb1258119) at dilution of 1:100 (40x lens).