MMP13 Rabbit Polyclonal Antibody

• Catalog Number: orb11057
• Category: Antibodies
• Description: MMP13 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MMP13 (201-300/471aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: MMP13
• UniProt ID: P45452
• RRID: AB_10748052
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CLG 3; CLG3; Collagenase 3; Collagenase3; MMP13; M
• Background: _CLIENT_OVERRIDE_
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse bone labeled with Anti-MMP-13 Polyclonal Antibody, Unconjugated (orb11057) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

MG63 (Human) Lysate at 30 ug, U 2OS (Human) Lysate at 30 ug, MDA-MB-231 (Human) Lysate at 30 ug, Primary: Anti-MMP13 (orb11057) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 60 kD.

Sample: Embryo (Mouse) Lysate at 40 ug, Primary: Anti-MMP13 (orb11057) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Sample: Ovary (Rat) Lysate at 40 ug, Primary: Anti-MMP13 (orb11057) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Tissue/Cell: human bladder carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated (orb11057) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.