MMP12 Antibody (C-term)

• Catalog Number: orb1930191
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB2026
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:2000, WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 54002 Da
• Target: This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12.
• UniProt ID: P39900
• NCBI: NP_002417.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Macrophage metalloelastase, MME, Macrophage elasta
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-MMP12 Antibody at 1:2000 dilution + human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of MMP12 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in 293 cell line lysate. MMP12 (arrow) was detected using the purified Pab;

Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in mouse kidney tissue lysates. MMP12 (arrow) was detected using the purified Pab.

Anti-MMP12 Antibody at 1:1000 dilution + human lung lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.