You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1294376 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to MMP 1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the center region |
Dilution range | Western Blot 1:500 Immunofluorescence 1: 100- 1:300 Immunohistochemistry (Paraffin) 1: 100-1:200 |
Conjugation | Unconjugated |
Target | MMP1 |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-MMP1 antibody at 1/500 dilution, Lysates/proteins at 40 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294376 overnight at 4°C.
All lanes: Anti-MMP1 antibody at 1/500 dilution, Lysates/proteins at 40 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294376 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294376 anti-MMP1 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294376 anti-MMP1 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded mouse testis labeling MMP1 with orb1294376 at 1/100.
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Primate, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating