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Catalog Number | orb1230591 |
---|---|
Category | Proteins |
Description | MMP-3 Recombinant Protein |
Form/Appearance | Lyophilized |
Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
Target | MMP3 |
UniProt ID | P08254 |
Protein Sequence | MRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PETPLVPTEP VPPEPGTPAN CDPALSFDAV STLRGEILIF KDRHFWRKSL RKLEPELHLI SSFWPSLPSG VDAAYEVTSK DLVFIFKGNQ FWAIRGNEVR AGYPRGIHTL GFPPTVRKID AAISDKEKNK TYFFVEDKYW RFDEKRNSME PGFPKQIAED FPGIDSKIDA VFEEFGFFYF FTGSSQLEFD PNAKKVTHTL KSNSWLNC |
Source | E. coli |
Biological Activity | MMP-3 activity was measured by its ability to cleave a chromogenic peptide MMP-3 substrate at room temperature. At a MMP-3 concentration of 2.5 μg/mL, 50% cleavage was achieved at an incubation time of approximately 75 minutes. |
NCBI | NP_002413.1 |
Storage | The lyophilized MMP-3 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted MMP-3 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing MMP-3 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
Alternative names | SL-1, STMY, STR1, CHDS6, MMP-3, STMY1, Stromelysin Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Greater than 85% as determined by SDS-PAGE. | |
48.5 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
Greater than 95% as determined by reducing SDS-PAGE. | |
53.26 kD | |
Human Cells |
> 92% by SDS-PAGE. | |
KMP1600, Recombinant Human MMP-3 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Tyr18-Cys477) of human MMP-3 (Accession #NP_002413.1) fused with a 6×His Tag at the C-terminus. |
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