MLF1 antibody

• Catalog Number: orb345628
• Category: Antibodies
• Description: MLF1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a 200 residue recombinant protein corresponding to the amino terminal end of human MLF1IP protein.
• Concentration: 0.88 mg/mL
• Dilution range: ELISA: 1:650,000, IHC: User Optimized, WB: 1:500- 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human MLF1IP protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, chimpanzee (98%), horse (72%), bovine (69%), dog (65%), mouse (56%), rat (55%) and chicken (47%) sources based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q71F23
• NCBI: 38016935
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-MLF1 antibody, rabbit anti-MLF1 Intera
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 65 kDa in size corresponding to MLF1IP protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of rabbit anti-MLF1 antibody. Tissue: brain, cerebellum. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-MLF1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: MLF1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of rabbit anti-MLF1 antibody. Tissue: heart. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-MLF1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: MLF1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Western blot using Biorbyt's affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1000.