MLCK Antibody (N-term)

• Catalog Number: orb1928724
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB03067
• Tested applications: WB
• Predicted Reactivity: Rabbit
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 210715 Da
• Target: This MLCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 923-953 amino acids from the N-terminal region of human MLCK.
• UniProt ID: Q15746
• NCBI: NP_444259.1, NP_444256.3, NP_444253.3, NP_444255.3, NP_444254.3, NP_444260.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Myosin light chain kinase, smooth muscle, MLCK, sm
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

MLCK Antibody (M1) western blot analysis in SK-BR-3 cell line lysates (35 ug/lane).This demonstrates the MLCK antibody detected the MLCK protein (arrow).

Western blot analysis of anti-MLCK-long Pab in mouse brain tissue lysate. MLCK-long (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35 ug.

Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35 ug.