MLANA Antibody

• Catalog Number: orb1252768
• Category: Antibodies
• Description: MLANA Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: M2-7C10
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG2b, kappa
• Immunogen: Recombinant human MART-1 protein was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MART-1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MLANA
• UniProt ID: Q16655
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A,
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MART-1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human melanoma stained with MART-1 antibody (M2-7C10). Note cytoplasmic staining of cells.

IHC testing of human melanoma stained with MART-1 antibody (M2-7C10). Note cytoplasmic staining of cells.

SDS-PAGE Analysis of Purified, BSA-Free MART-1 Antibody (clone M2-7C10). Confirmation of Integrity and Purity of the Antibody.