MitoPT JC-1 Assay Kit (400 tests)

• Catalog Number: orb1409962
• Category: Tools
• Description: JC-1 accumulates and fluoresces orange in polarized mitochondria. Following membrane depolarization, JC-1 disperses within the cell and fluoresces green. Analyze using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.
• Target: Mitochondrial depolarization
• Storage: -20°C
• Note: For research use only
• Application notes: 1. Prepare samples and controls, 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20., 3. Reconstitute FLICA with 50 μL DMSO., 4. Dilute FLICA 1:5 by adding 200 μL PBS., 5. Add diluted FLICA to each sample at 1:30 (e.g., add 10 μL to 290 μL of cultured cells)., 6. Incubate approximately 1 hour., 7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells., 8. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody., 9. If desired, fix cells., 10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM FLICA excites at 492 nm and emits at 520 nm., , If working with adherent cells, please see the manual for additional protocols.
• Sample Types: Cell culture
• Expiration Date: 12 months from date of receipt.