Mitochondrial Marker Antibody

• Catalog Number: orb606937
• Category: Antibodies
• Description: This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MTC02/2860R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A semi-purified mitochondria preparation was used as the immunogen for this recombinant Mitochondrial Marker antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Mitochondrial Marker antibody is available for research use only.
• UniProt ID: Unknown
• Storage: Store the recombinant Mitochondrial Marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Mitochondrial Marker antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human pancreas with recombinant Mitochondrial Marker antibody (clone MTC02/2860R). HIER: boil tissue sections in 1mM EDTA buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT prior to testing.

SDS-PAGE analysis of purified, BSA-free recombinant Mitochondrial Marker antibody (clone MTC02/2860R) as confirmation of integrity and purity.