MITF (phospho-S180) antibody

• Catalog Number: orb515489
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MITF (phospho-S180)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S180 of human MITF protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:50-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MITF
• Entrez: 17342, 4286
• UniProt ID: O75030, Q08874
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: BHLHE32; Microphthalmia-associated transcription f
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MITF (Phospho-S180) expression in U87MG (A), A549 (B) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 52 kD)

Immunohistochemical analysis of MITF (Phospho-S180) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-MITF (Phospho-S180) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.