MITF antibody

• Catalog Number: orb1151878
• Category: Antibodies
• Description: Mouse monoclonal antibody to MITF
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-MITF-1D9
• Tested applications: FACS, IF, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human MITF protein was used as the immunogen for the MITF antibody.
• Dilution range: Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This MITF antibody is available for research use only.
• UniProt ID: O75030
• Storage: Aliquot the MITF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the MITF antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

FACS staining of PFA-fixed human HeLa cells with MITF antibody (blue, clone PCRP-MITF-1D9) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free MITF antibody (PCRP-MITF-1D9) as confirmation of integrity and purity.

Immunofluorescent staining of PFA-fixed human HeLa cells using MITF antibody (green, clone PCRP-MITF-1D9) and phalloidin (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using MITF antibody (clone PCRP-MITF-1D9). These results demonstrate the foremost specificity of the PCRP-MITF-1D9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.