MIP-3 alpha (RAT) antibody (Biotin)

• Catalog Number: orb345245
• Category: Antibodies
• Description: MIP-3 alpha (RAT) antibody (Biotin)
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 4N5F7
• Tested applications: ELISA, IP, NeA, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Anti-MIP-3α (RAT) Monoclonal Antibody was produced in rat by repeated immunizations with mature full length recombinant mouse MIP-3α produced in E.coli followed by hybridoma development.
• Concentration: 1.0 mg/mL
• Dilution range: WB: 0.5µg/mL
• Form/Appearance: Lyophilized
• Purity: This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse MIP-3α protein. A BLAST analysis was used to suggest cross-reactivity with MIP-3α from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with MIP-3α from other sources has not been determined.
• Conjugation: Biotin
• UniProt ID: O89093
• NCBI: NP_001153210.1
• Storage: Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rat anti-MIP-3α biotin conjugated antibody, CCL20,
• Note: For research use only
• Application notes: This purified antibody has been tested for use in ELISA, IP, western blot, and Neutralization. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Mouse MIP 3α (RAT) antibody. Lane 1: mouse recombinant MIP-3a. Lane 2: human recombinant MIP-3a. Primary antibody: MIP 3α antibody at 0.1 ug/ml for overnight at 4°C. Secondary antibody: IRDye800™ goat anti-rat at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.