MIP-3 alpha Biotin Conjugated Antibody

• Catalog Number: orb345118
• Category: Antibodies
• Description: MIP-3 alpha antibody (Biotin)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3N2D9
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-MIP-3α (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human MIP-3α produced in E.coli followed by hybridoma development.
• Concentration: 1.0 mg/mL
• Dilution range: WB: 0.5µg/mL
• Form/Appearance: Lyophilized
• Purity: This product was purified from mouse ascites by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human MIP-3α protein. A BLAST analysis was used to suggest cross-reactivity with MIP-3α from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with MIP-3α from other sources has not been determined.
• Conjugation: Biotin
• UniProt ID: P78556
• NCBI: 4759076
• Storage: Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-MIP-3 alpha Antibody biotin Conjugated,
• Note: For research use only
• Application notes: This purified antibody has been tested for use in Western Blot. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Unconjugated Human MIP 3α (Mouse) Antibody. Lane 1: human recombinant MIP-3a. Lane 2: mouse recombinant MIP-3a. Primary antibody: Human MIP 3α (Mouse) Antibody (orb345108) at 0.5 ug/ml for overnight at 4°C. Secondary antibody: IRDye800™ goat anti-mouse at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.