MIP-3 alpha antibody (Biotin)

• Catalog Number: orb345177
• Category: Antibodies
• Description: MIP-3 alpha antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: MIP-3 alpha Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3α protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Form/Appearance: Lyophilized
• Purity: Human MIP-3 alpha Antibody Biotin Conjugated was heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human MIP-3α in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
• Conjugation: Biotin
• UniProt ID: P78556
• NCBI: 4759076
• Storage: Store MIP-3 alpha Antibody Biotin Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-MIP-3 alpha Antibody Biotin Conjugated
• Note: For research use only
• Application notes: This purified antibody has been tested in western blotting and is suitable in ELISA. By western blot a band approximately 11 kDa in size corresponding to human MIP-3α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Biotin Conjugated Rabbit Anti-Human MIP-3α Antibody. Lane 1: Human MIP-3α. Lane 2: None. Load: 50 ng per lane. Primary antibody: Human MIP-3α antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 10 kDa, 10 kDa for Human MIP-3α. Other band(s): None.