MIP 1 beta antibody

• Catalog Number: orb345257
• Category: Antibodies
• Description: MIP 1 beta antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Porcine
• Isotype: IgG
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein raised in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP1ß protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: >1:5,000, WB: 1:500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for swine CCL4 protein. A BLAST analysis was used to suggest cross-reactivity with CCL4 from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4 based on 90% homology. Cross-reactivity with CCL4 from other sources has not been determined. The swine CCL4 sequence is also 86% homologous to swine CCL3L1.
• Conjugation: Unconjugated
• UniProt ID: Q711P4
• NCBI: ABL74287
• Storage: Store vial at 4° C upon receipt. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-MIP 1 beta Antibody, rabbit anti-MIP-1
• Note: For research use only
• Application notes: This protein-A purified MIP-1ß antibody has been tested by western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kDa in size corresponding to swine CCL4 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's protein-A purified Anti-MIP-1ß (CCL4) antibody shows detection of recombinant swine MIP-1ß (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (diluted 1:10), and probed with 4 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800™ Conjugated Goat Anti-Rabbit IgG at 1:20000 for 45 min at room temperature.