MIP-1 alpha antibody

• Catalog Number: orb345216
• Category: Antibodies
• Description: MIP-1 alpha antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse MIP-1α protein produced in E.coli.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000, WB: 1:1000
• Form/Appearance: Lyophilized
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse MIP-1α protein. Cross-reactivity with MIP-1α from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P10855
• NCBI: NP_035467.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: rabbit anti-MIP-1α antibody, rabbit anti-MIP-1 alp
• Note: For research use only
• Application notes: This purified antibody has been tested in western blotting and is suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7-8 kDa in size corresponding to the mature 92 amino acid mouse MIP-1α/Ccl3 protein by western blotting in appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Anti-mouse MIP-1a in western blot shows detection of recombinant mouse MIP-1a/Ccl3 raised in E.coli. Recombinant truncated (0.1 µg, expect ~8 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Biorbyt's Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20000 in blocking buffer (p/n orb348637) for 30 min. at RT.