Milk Fat Globule Antibody (MFG-E8, Lactadherin)

• Catalog Number: orb749665
• Category: Antibodies
• Description: Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin, or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MFG-06
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A human milk fat globule membrane preparation was used as the immunogen for the MFGE8 antibody.
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This MFGE8 antibody is available for research use only.
• UniProt ID: Q08431
• Storage: Store the MFGE8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the MFGE8 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with MFGE8 antibody (clone MFG-06).

IHC analysis of FFPE human pancreas tested with MFGE8 antibody (MFG-06)

IHC analysis of FFPE human lung carcinoma tested with MFGE8 antibody (MFG-06)

IHC analysis of FFPE human renal cell carcinoma tested with MFGE8 antibody (MFG-06)

Western blot testing of MCF7 cell lysate with MFGE8 antibody (clone MFG-06).

SDS-PAGE Analysis of Purified, BSA-Free MFGE8 Antibody (clone MFG-06). Confirmation of Integrity and Purity of the Antibody.